Department of
LIFE-SCIENCES






Syllabus for
Master of Science in Biotechnology
Academic Year  (2019)

 
1 Semester - 2019 - Batch
Paper Code
Paper
Hours Per
Week
Credits
Marks
MLIF136 RESEARCH METHODOLOGY IN BIOLOGICAL SCIENCES 2 2 50
MLIF152 PRACTICAL IN CELL BIOLOGY AND GENETICS 8 4 100
2 Semester - 2019 - Batch
Paper Code
Paper
Hours Per
Week
Credits
Marks
MBTY251 PRACTICAL IN MOLECULAR BIOLOGY AND PLANT BIOTECHNOLOGY 8 4 100
MLIF232 GENETIC ENGINEERING 4 4 100
MLIF251 PRACTICAL IN GENETIC ENGINEERING, BIOANALYTICAL TECHNIQUES AND BIOINFORMATICS 8 4 100
3 Semester - 2018 - Batch
Paper Code
Paper
Hours Per
Week
Credits
Marks
MBTY331 BIOPROCESS ENGINEERING 4 4 100
MBTY351 PRACTICAL IN IMMUNOLOGY AND BIOPROCESS ENGINEERING 8 4 100
MBTY352 PRACTICAL IN PLANT BIOTECHNOLOGY AND ANIMAL BIOTECHNOLOGY 8 4 100
MLIF331 PLANT BIOTECHNOLOGY AND REGULATORY AFFAIRS 4 4 100
MLIF332 IMMUNOLOGY 4 4 100
MLIF381 SUMMER INTERNSHIP 0 2 50
4 Semester - 2018 - Batch
Paper Code
Paper
Hours Per
Week
Credits
Marks
MBTY441B ENVIRONMENTAL BIOTECHNOLOGY 4 4 100
MBTY441C DISEASE BIOLOGY 4 4 100
MBTY452A PRACTICAL IN FOOD TECHNOLOGY 4 2 50
MBTY452B PRACTICAL IN ENVIRONMENTAL BIOTECHNOLOGY 4 2 50
        

  

Assesment Pattern

Evaluation will be done on the basis of CIA1 (10%), CIA2 [Mid Semester Examination] (25%), CIA3 (10%), Attendance (5%) and End Semester Examination (50%).

CIA1: Assignment/test/poster preparation/review writing etc. for 20 marks

CIA2: MID SEMESTER EXAMINATION for 50 marks

CIA3: Assignment/test/poster preparation/review writing etc. for 20 marks

Attendance in class: 10 marks

END SEMSTER EXAMINATION: Consist of 2 sections. Section A consist of 10 questions carrying 5 marks each out of which students need to attempt 8 questions (8 X 5marks = 40 marks). Section B consists of 7 questions, carrying 12 marks each, out of which students need to attempt 5 questions (5 X 12 marks = 60 marks).

Examination And Assesments

The evaluation scheme for each course shall contain two parts; (a) internal evaluation and (b) external evaluation. 50% weightage shall be given to internal evaluation and the remaining 50% to external evaluation and the ratio and weightage between internal and external is 1:1.  (a) Internal evaluation: The internal evaluation shall be based on predetermined transparent system involving periodic written tests, assignments, seminars and attendance in respect of theory courses and based on written tests, lab skill/records/viva and attendance in respect of practical courses.

Department Overview:
The department of Life Sciences is a unique department in the University where multidisciplinary and interdisciplinary teaching and research in life sciences have established permanent roots. It is a diverse discipline that covers all branches of Zoology, Botany and Biotechnology in a dominant manner. This is one of the oldest departments of Christ University (formerly Christ College) started from the inception of the Institution in 1969. This serves as a valuable foundation to many students for understanding cellular and molecular level organization in living beings. The uniqueness of the department essentially lies in the fact that within its faculty there are experts and active researchers representing almost all areas of modern biology. Two research laboratories have been set under the department of Life Sciences in Kengeri Campus to promote the research in plant Science. Phytochemistry and Pharmacognosy Research Laboratory focuses on the extraction, purification, characterization and identification of secondary metabolites present in plants. Particularly, we focus on the secondary metabolites of medicinal plants like Andrographis paniculata, Centella asiatica, Nothapodytes etc. Plant Tissue Culture laboratory focuses on developing biotechnological approaches for the production of secondary metabolites from medicinal plants. We also aim at the rapid multiplication of medicinal plants through plant tissue culture in this laboratory.
Mission Statement:
To uphold the core values of the university and to build up a ?Life Science Community,? for the betterment of humanity with their knowledge, ethics and entrepreneurship.
Introduction to Program:
Biotechnology is a fundamental area of applied science that utilizes living cells and cellular materials to create pharmaceutical, diagnostic, agricultural, environmental, and other products to benefit society. The Master of Science in Biotechnology is designed to provide specialized scientific learning along with skills training to help students explore various career paths in agriculture, health care, forensics, industrial processing, and environmental management. Students will be provided hands on learning into the functioning of the biotechnology industry. Students will have to undertake an Industry Project in their second year of the programme.
Program Objective:
To develop a detailed technical understanding of the key methods used in the contemporary biotechnology sector; To appreciate the techniques applied in biotechnology and advanced research; To acquire and critically appraise new data arising from the use of these techniques and to interpret the implications of such data; To develop an understanding of the commercial, financial and regulatory context in which the biotechnology sector operates.

MLIF136 - RESEARCH METHODOLOGY IN BIOLOGICAL SCIENCES (2019 Batch)

Total Teaching Hours for Semester:30
No of Lecture Hours/Week:2
Max Marks:50
Credits:2

Course Objectives/Course Description

 

1.      To understand the theoretical basis of conducting research

2.      To design a research

3.      Understanding the importance of the research paper

4.      To impart knowledge regarding the ethics in research

Learning Outcome

By the end of this course, students will be able to

·         find gaps in the existing research of their interest and conduct the research accordingly

·         to write a research proposal.

·         publish research and review articles in the journal with impact factor.

·         write a project report as well as research paper.

Unit-1
Teaching Hours:10
Concepts of Research and Research Formulation
 

Need for research, stages of research; Basic concepts of research -Meaning, Objectives, Motivation and Approaches. Types of Research (Descriptive/Analytical, Applied/ Fundamental, Quantitative/Qualitative, Conceptual/ Empirical); Research formulation -Observation and Facts, Prediction and explanation, Induction, Deduction; Defining and formulating the research problem, Selecting the problem and necessity of defining the problem; Literature review -Importance of literature reviewing in defining a problem, Critical literature review, Identifying gap areas from literature review; Hypothesis -Null and alternate hypothesis and testing of hypothesis -Theory, Principle, Law and Canon.

Unit-2
Teaching Hours:7
Research Designs
 

Research Design -Basic principles, Meaning, Need and features of good design, Important concepts; Types of research designs; Development of a research plan -Exploration, Description, Diagnosis, Experimentation, determining experimental and sample designs; Data collection techniques, Case-Control Studies, Cohort Studies.

Unit-3
Teaching Hours:4
Scientific Documentation and Communication
 

Workbook maintenance, Project proposal writing, Research report writing (Thesis and dissertations, Research articles, Oral communications); Presentation techniques - Assignment, Seminar, Debate, Workshop, Colloquium, Conference.

Unit-4
Teaching Hours:9
Information Science, Extension and Ethics
 

Sources of Information -Primary and secondary sources; Library - books, Journals: Indexing journals, abstracting journals, research journals, review journals, e-journals. Impact factor of journals, NCBI-Pub Med.; periodicals, reference sources, abstracting and indexing sources, Reviews, Treatise, Monographs, Patents. Internet -Search engines and software, Online libraries, e-Books, e-Encyclopedia, TED Talk, Institutional Websites; Intellectual Property Rights - Copy right, Designs, Patents, Trademarks, plagiarism, Geographical indications; Safety and precaution - ISO standards for safety, Lab protocols, Lab animal use, care and welfare, animal houses, radiation hazards; Extension: Lab to Field, Extension communication, Extension tools; Bioethics: Laws in India, Working with man and animals, Consent, Animal Ethical Committees and Constitution.

Text Books And Reference Books:

1.  Thomas, C.G., Research Methodology and Scientific Writing. Anne Books Pvt. Ltd. Bengaluru. 2017.

      2.      Dawson, C. Practical research methods. UBS Publishers, New Delhi. 2002.

Essential Reading / Recommended Reading

1.      Stapleton, P., Yondeowei, A., Mukanyange, J., Houten, H.  Scientific writing for agricultural research scientists – a training reference manual. West Africa Rice Development Association, Hong Kong, 1995.

2.      Ruzin, S.E. Plant micro technique and microscopy. Oxford University Press, New York, U.S.A., 1999.


 

Evaluation Pattern

Evaluation will be based on 10% CIA 1, 25% CIA 2, 10% CIA 3 and 5% Attendance

MLIF152 - PRACTICAL IN CELL BIOLOGY AND GENETICS (2019 Batch)

Total Teaching Hours for Semester:120
No of Lecture Hours/Week:8
Max Marks:100
Credits:4

Course Objectives/Course Description

 

The paper imparts practical knowledge on the biology of cells and also on the basic experiments in biochemistry. It deals with detailed microscopic studies of basic cell multiplication processes like mitosis and meiosis. Microscopy techniques are given utmost importance.   Furthermore, knowledge of Genetics will help them to solve various complicated genetic problems.

Learning Outcome

The students gain expertise in observing cells and processes like mitosis and meiosis under microscope, which in turn will help them work better in clinical laboratories. Furthermore, the students will learn the importance of cell fractionation. Students will also learn various aspects of Genetic experiments

Unit-1
Teaching Hours:60
Cell Biology
 

 

      Study of mitosis in root tip

 

      Study of meiosis of flower bud

 

      Study of Plasmolysis- deplasmolysis using micrometry

 

      Study of Barr body in the epithelial cells

 

      Isolation and enumeration of mitochondria from yeast cells

 

      Isolation and enumeration of chloroplast from spinach

 

      Estimation of chlorophyll in isolated chloroplasts

 

      Comparative study of chloroplast number and chlorophyll content in different plant families

 

      Permanent slide preparation

 

Unit-2
Teaching Hours:60
Genetics
 

      Genetic Problems in Recombination and Linkage

      Genetic problems in quantitative genetics

      Genetic problems in population genetics

      Genetic problems in pedigree analysis

Text Books And Reference Books:

J. E. Celis, Cell Biology: A laboratory Hand Book, 3rded. USA: Elsevier Academic Press, 2006.

Essential Reading / Recommended Reading

J. E. Celis, Cell Biology: A laboratory Hand Book, 3rded. USA: Elsevier Academic Press, 2006.

Evaluation Pattern

CIA Evaluation:

Performance: 40 marks

Mid Semester Examination: 40 marks

Record: 20 marks

 

 

End Semester Examination:

 

Time: 6 Hours                                                                     Total Marks: 100

1.      Isolation of chloroplast and estimation of chlorophyll content from the given sample                                                                                                (20 marks)

 

OR

 

Isolation and enumeration of chloroplast from the given sample

 

OR

 

Isolation and enumeration of mitochondria from yeast.

 

(Introduction: 2 marks; Principle: 4 marks; Procedure: 4 marks; Results and discussion: 10 marks)                                   

 

 

 

2.      Preparation of buccal smear for the study of Barr body                   (15 marks)

 

OR

 

Prepare temporary squash of the given biological sample and report any two stages of mitosis.

 

OR

 

Prepare temporary squash of the given biological sample and report any two stages of meiosis.

 

(Introduction: 2 marks; Principle: 3 marks; Procedure: 2 marks; Results and discussion: 8 marks)

 

 

 

 

 

3.      Logical Reasoning                                                                 (3 X 3 marks = 9 marks

 

 

 

4.      Spotters                                                                                (4 X 4 marks = 16 marks)

 

 

 

5.      Viva                                                                                                    (10 marks)

 

 

 

6.      Problems in Genetics                                                                   (30 marks)

 

MBTY251 - PRACTICAL IN MOLECULAR BIOLOGY AND PLANT BIOTECHNOLOGY (2019 Batch)

Total Teaching Hours for Semester:120
No of Lecture Hours/Week:8
Max Marks:100
Credits:4

Course Objectives/Course Description

 

Practical course of molecular biology deals with the isolation of protein from animal and plant source using different methods and also the estimation of DNA, RNA and protein as well as they will understand various applications of plant biotechnology.

Learning Outcome

By the end of the course the students will be familiar with different molecular biological techniques like electrophoresis, chromatography, spectrophotometry and also the students now know how to estimate the amount of macromolecules like DNA, RNA and proteins.

Students will learn various techniques in molecular biology like DNA and RNA estimation, Bioinofrmatics like docking, constructing dendogram, tools (BLAST, FASTA) etc. and Plant Biotechnology like aseptic handling of plant materials, culture of callus, protoplasts etc.

Unit-1
Teaching Hours:120
Practical in Molecular Biology, Bioinformatics and Genetics
 

Molecular Biology

      DNA and RNA estimation by colorimetry and spectrophotometry

      Screening of auxotrophic mutants

      Bacteriophage assay

      UV and chemical mutagenesis

      Screening of markers

      Conjugation mapping

 

 

Plant Biotechnology

   

·         Introduction to Plant Tissue Culture Laboratory
Nutritional components of Tissue Culture Media

 

·         Introduction to aseptic techniques
Introduction to different types of explants
Surface sterilization techniques
Callus initiation and Maintenance

 

·         Initiation of Cell suspension cultures and Growth kinetic studies using PCV/fresh and Dry weight basis

 

·         Induction of multiple shoots by using different explants

 

·         Culturing of anthers for the induction of haploids

 

·         Isolation and Culturing of protoplast

 

·         Induction of hairy roots with Agrobacterium rhizogenes infection using different explants and confirmation of transgene using PCR

 

·         Production of Artificial seeds using sodium alginate

 

·         Induction of Somatic Embryogenesis

 

       

 

 
 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Text Books And Reference Books:

S Sadasivam, A. Manickam. Biochemical Methods. 2Ed, Delhi: New Age International Publishers Ltd, 1996.

Essential Reading / Recommended Reading

S. K. Sawhney. R. Singh. Introductory Practical Biochemistry. New Delhi. Narosa Publications. 2014.

Evaluation Pattern

CIA Evaluation

Performance: 40 marks

Mid Semester Examination: 40 marks

Record: 20 marks

End Semester examination pattern:

1.      1. Conduct the experiment

(Total Marks 35 = Principle, procedure and graph, if any – 10, Working – 15, Result – 5, Comments/Interpretation - 5)

2.      2. Prepare the given explant for inoculation

(Total Marks 8 = Principle-2, Procedure-2, Working & Result: 4)                                                 

3.      3. Prepare synthetic seeds using the given plant material

(Total Marks 8 = Principle-2, Procedure-2, Working & Result: 4)                                                                            

4.      4. Submission of responding culture

  (Total Marks 8 = 2 X 4)                                                                                                                                    

5.     5. Logical questions

(Total Marks 6 = 2 x 3)                                                                                                                                                

6.     6. Spotters                                                                         

(Total Marks 10 = 4 x 2.5)

7. Estimation of DNA/RNA using colourimetrty/Spectrophotometry with calculations (Total Marks 25: Principle 5 marks, Procedure 5 marks, Results 15 marks)

 

MLIF232 - GENETIC ENGINEERING (2019 Batch)

Total Teaching Hours for Semester:60
No of Lecture Hours/Week:4
Max Marks:100
Credits:4

Course Objectives/Course Description

 

The objective of the course is to impart in depth knowledge about the concepts in genetic engineering - enzymes, biology of cloning vehicles, vector and host considerations, gene libraries, analysis and expression of the cloned gene in host cell and understand ethical issues and biosafety regulations. It gives emphasis to practical applications of genetic engineering tools in academic and industrial research. At the end of the course the student will have detailed knowledge of recombinant DNA technology essential for taking up projects in the field of Biotechnology.

Learning Outcome

By the end of this course, the students will have in-depth knowledge about different techniques used in rDNA technology, different methods of generating recombinant DNA, different types of vectors, host, methods and means of making of rDNA molecules and analysing them, fingerprinting and more over the students will have the idea about the application of genetic engineering and the biosafety and ethics related to such experiments.

Unit-1
Teaching Hours:20
Tools to Make rDNA
 

Introduction to rDNA technology, DNA modifying enzymes and its functions (DNA Polymerases, Klenow fragment, Ligase, S1 Nuclease, Mung Bean nuclease, Alkaline Phosphatase, Terminal Transferase, Polynucleotide kinases, Polynucleotide phosphorylase, Calf intestinal alkaline Phosphatases, Shrimp Alkaline Phosphatases, RNase A, RNase H, DNase 1, DNase II, Exonuclease III, Reverse Transcriptase) Restriction modification system, Restriction enzymes – function, classification (Based on recognition and restriction sequence:-type I, II and III; based on buffer salt concentration: - low, medium and high; based on pattern of restriction:-sticky (5’ and 3’) and blunt end cutters, Plasmids (Types, copy number, properties, origin of replication and incompatibility group, plasmid amplification), bacteriophages eg. λ (Life cycle, genome organization, feasibility as a cloning vehicle), Types of Cloning Vectors (structure and general features of General Purpose cloning vectors, Expression vectors, Promotor probe Vectors, shuttle vectors), Examples of cloning vectors (pBR322, pUC series of vectors, λ insertional and replacement vectors), derivatives of phages and plasmids (cosmids, phagemids, phasmids) cloning vectors for large DNA fragments and genomic DNA library YACs, PACs and BACs. Host and vector consideration, Host Organisms and its genotypes- JM 109 & DH5α, Selectable and scorable markers, reporter genes, prokaryotic and eukaryotic markers (lacZ, CAT, Gus, GFP,cre-loxP system, sac B system, npt II gene, luciferase gene, dhfr gene, herbicide resistance gene)

Unit-2
Teaching Hours:8
Making of rDNA Molecule
 

General strategies for isolation of genomic and plasmid DNA, RNA, strategies for isolation of gene of interest (restriction digestion, PCR), Creation of r-DNA (Restriction Digestion, modification of vector and insert, linker, adaptors, homopolymer tailing, ligation), PCR Cloning, Construction of genomic and cDNA libraries (Selection of vectors and Complexity of library), Methods of gene transfer- Calcium chloride mediated, Electroporation, Biolistic gun, lipofection and microinjection. In vitro packaging.

Unit-3
Teaching Hours:10
Screening and analysis of rDNA molecules
 

Blotting techniques- Southern, Northern and Western, Differential display. Gene sequencing- Chemical, enzymatic, pyrosequencing, next generation sequencing, Immunological screening and colony and plaque hybridization, dot blot hybridization, chromosome walking, FISH, RACE, Chromosome walking.

Unit-4
Teaching Hours:10
Expression & control of Genes
 

Protein production by foreign DNA in the host bacteria E. coli, Factors influencing expression, properties of expression vector, examples of expression vectors, tags for purification of expressed proteins, FLAG expression vector system, cloning in pET vectors, eukaryotic vectors- Baculovirus based vectors, mammalian viral vectors., expression Host, Modification and folding of protein in-vitro, genome editing, CRISPR/Cas9 and Targeted Genome Editing, 

Unit-5
Teaching Hours:12
Applications of r-DNA Technology
 

RNA interference and gene silencing, Transgenic organisms, Advantages and disadvantages of Genetically Modified Organisms, Transgenic animal- Gene therapy. The Use of Transgenic animals in areas other than recombinant protein production. Transgenic plants- applications, special emphasis to pharmaceutical products. Engineered Nutritional Changes- golden rice, Engineered herbicide resistance, Engineered pesticide resistance. Production of recombinant proteins (Insulin), recombinant vaccines (Hepatitis B), Hormones (Human growth hormone). Genome projects and its Applications. International treaties/agreements in biosafety, public perception on rDNA technology, IPR related to rDNA technology. 

Text Books And Reference Books:

M. L. Srivastava, Bioanalytical Techniques, New Delhi: Narosa Publications, 2011.

E. L. Winnacker, From Genes to Clones Introduction to Gene Technology,New Delhi, India: Panima Publishing Corporation, 2003.

 

T. A. Brown, Gene Cloning and DNA Analysis-An Introduction. 5th ed. UK: Wiley Blackwell Publishers. 2006.

Essential Reading / Recommended Reading

Alkami Quick Guide for PCR A laboratory reference for the Polymerase Chain Reaction, USA. Alkami Biosystems Inc., 1999.

B. R. Glick. J. J. Pasternak and C. L. Patten. Molecular Biotechnology: Principles and application of recombinant DNA. 4th ed. Washington D. C: American Society for Microbiology Press, 2010.

 S. B. Primrose, R. M. Twyman and R. W. Old, Principles of Gene Manipulation, 6th ed. USA: Wiley-Blackwell, 2001

K. Wilson and J. Walker, Principles and Techniques of Biochemistry and Molecular Biology, 7th ed. New York: Cambridge University Press, 2010.

 

 J.  W.  Dale, M. von Schantz and N. Plant, From Genes to Genomes: Concepts and Applications of DNA Technology, USA: John Wiley & Sons Inc., 2012.

Evaluation Pattern

Evaluation will be done on the basis of CIA1 (10%), CIA2 [Mid Semester Examination] (25%), CIA3 (10%), Attendance (5%) and End Semester Examination (50%).

CIA1: Assignment/test/poster preparation/review writing etc. for 20 marks

CIA2: MID SEMESTER EXAMINATION for 50 marks

CIA3: Assignment/test/poster preparation/review writing etc. for 20 marks

Attendance in class: 10 marks

END SEMSTER EXAMINATION: Consist of 2 sections. Section A consist of 10 questions carrying 5 marks each out of which students need to attempt 8 questions (8 X 5marks = 40 marks). Section B consists of 7 questions, carrying 12 marks each, out of which students need to attempt 5 questions (5 X 12 marks = 60 marks).

MLIF251 - PRACTICAL IN GENETIC ENGINEERING, BIOANALYTICAL TECHNIQUES AND BIOINFORMATICS (2019 Batch)

Total Teaching Hours for Semester:120
No of Lecture Hours/Week:8
Max Marks:100
Credits:4

Course Objectives/Course Description

 

The aim of this course is to provide an introduction to recombinant DNA technology. It helps the students to understand how the principles of molecular biology have been used to develop techniques in recombinant DNA technology. The objective of the course is to familiarize the student with the basic concepts in genetic engineering - enzymes, cloning vehicles, gene libraries, analysis and expression of the cloned gene in host cell and understand ethical issues and biosafety regulations. It gives emphasis to practical applications of genetic engineering tools in the field of health care. At the end of the course the student will have enough background of recombinant DNA technology essential for taking up projects in the field of Biotechnology.

Learning Outcome

Modern Biotechnology relies on rDNA technology. This paper will equip the student with all the basic rDNA methods and protocols. By the end of the course, the students will be
familiar with and gain hands on training on basic rDNA methodologies. Moreover, the students will be able to find a job in R&D laboratories/industries where rDNA works are being done.

Unit-1
Teaching Hours:60
Genetic Engineering
 

1

Isolation and purification of DNA from plant, animal, bacterial and fungal samples.

2

Isolation of plasmid DNA from the bacteria.

3

Isolation of megaplasmid from the environmental isolates.

4

Isolation of RNA from plant, animal and bacterial samples and separation on denaturing gel.

5

Primer design and PCR amplification of DNA.

6

Gel-band purification for DNA.

7

RFLP and RAPD, ISSR/SSR analysis of DNA

8

Cloning and expression of gene in E. coli.

9

Southern blotting and hybridization.

10

Agarose gel electrophoresis.

11

Study of star activity of restriction Enzyme.

12

Study of complete and partial digestion of DNA.

13

Effect of different parameters on Restriction digestion.

Site Directed Mutagenesis

 
 
 
 
 
 
 
 
 
 
 
 
Unit-2
Teaching Hours:30
Bioanalytical Techniques
 

14

Analysis of Amino Acids and Sugars (TLC and Colorimetric)

15

Extraction of phytochemicals using Soxhlet apparatus

16

Column Chromatography

17

Affinity chromatography.

18

HPLC- Principle and sample preparation, visit to Research Institute for analysis

19

Zymogram

20

Microwave assisted extraction

21

Density Gradient Centrifugation

22

Dialysis and purification of proteins

23

Isoelectric focusing

24

Colorimetry and spectrophotometry

   
Unit-3
Teaching Hours:30
Bioinformatics
 

Docking studies of ligands

Construction of dendogram

Pubmed

BLASTN, BLASTP and BLASTX

FASTA

KEGG

EXPASY

RasMol

Text Books And Reference Books:

S Sadasivam, A. Manickam. Biochemical Methods. 2Ed, Delhi: New Age International Publishers Ltd, 1996.

Essential Reading / Recommended Reading

S. K. Sawhney. R. Singh. Introductory Practical Biochemistry. New Delhi. Narosa Publications. 2014.

Evaluation Pattern

CIA Evaluation

Performance: 40 marks

Mid Semester Examination: 40 marks

Record: 20 marks

End Semester Examination

MAXIMUM MARKS: 100                                                               DURATION: 6 HOURS 

 

Sl No.

Question

Marks

1

Isolation and purification of gDNA/Plasmid and analyze on agarose gel (Principle 5 marks, Procedure 5 marks, Results 20 marks)

30

2

SDS PAGE/ Column Chromatography (Principle 5 marks, Procedure 5 marks, Results 20 marks)

30

3

Construct a phylogenetic tree using the following nucleotide sequences from different species.                                                                                                             

10

4

Protein/Nucleotide Sequence Alignment using BLAST OR Protein structure studies by Rasmol  OR Gene sequence search using online tools.                                                                                               

5

5

Spotters 5 X 4 Marks

15

6

Viva

10

   
     
     
     
     

 

MBTY331 - BIOPROCESS ENGINEERING (2018 Batch)

Total Teaching Hours for Semester:60
No of Lecture Hours/Week:4
Max Marks:100
Credits:4

Course Objectives/Course Description

 

This paper covers important topics in the development, production, recovery, and analysis of products produced by biotechnology. The course traces the path of a biological product from the cell through the production facility, the final processing, and formulation. It discusses the growth characteristics of the organisms used to produce biological compounds, the techniques used in product recovery and purification analysis. The course emphasizes the use of Good Manufacturing Practices (GMP) in these analyses. 

Learning Outcome

Students learn and understand cell growth, goals of fermentation and relate it to a real-life example. Different types of bioreactor, auxiliary equipment and applications are understood. Students understand how parameters such as pH, temperature, aeration, and agitation that affects the fermentation. Students learn the industrial production of certain metabolites and their recovery. Students learn and assess biological methods for pollution control, energy and resource recovery from waste, bioremediation and how they can contribute to clean technology, Biological processes to remove or recover inorganic and organic pollutants from liquid and gaseous effluents, and from solid waste, Biological processes for energy and biomass production from waste.

Unit-1
Teaching Hours:9
Introduction to Bioprocess Engineering and Fermentation
 

Definition of a Bioprocess, over view of bioprocesses with their various components. Bioprocess operation & their global impact. Fermentation process- inoculum build up, pre-fermentation, product fermentation. Solid state fermentation, Solid substrate, submerged, Aerobic, Anaerobic, batch, fed-batch, semi-continuous, continuous, Fermentation based on type of product formation- type I, II, III. 

Unit-2
Teaching Hours:7
Bioreactor
 

Types of Bioreactors- conventional- stirred tank reactors, airlift, bubble up fluidized bed, packed bed, tower reactors, drum reactors, photobioreactors; Components of the fermenters, types of impellers, aeration, temperature regulation, pH monitoring, antifoaming agents.

Unit-3
Teaching Hours:6
Media design and Sterilization Kinetics
 

Criteria for good medium, medium requirements for fermentation processes, carbon, nitrogen, minerals, vitamins and other complex nutrients, oxygen requirements, medium formulation of optimal growth and product formation, examples of simple and complex media, design of various commercial media for industrial fermentations – medium optimization methods Thermal death kinetics of microorganisms, batch and continuous heat sterilization  and filter sterilization of liquid media, Air sterilization

Unit-4
Teaching Hours:10
Kinetics of microbial growth and Culture Management
 

Isolation, screening and strain improvement (mutation, selection of mutants, selective isolation of mutants- genetic recombination) of microbes, Type culture collection, preservation of microbes. Modes of operation - batch, fed batch and continuous cultivation. Simple unstructured kinetic models for microbial growth, Monod model, growth of filamentous organisms, product formation kinetics - leudeking-piret models, substrate and product inhibition on cell growth and product formation 

Unit-5
Teaching Hours:8
Downstream Processing
 

Solid-liquid separation (Flocculation, Filtration, Centrifugation), Cell disruption (Physical, chemical and enzymatic), Extraction, Precipitation, Distillation, Evaporation, Chromatographic separation, Adsorption, Concentration, formulation- Lyophilisation, spray drying

Unit-6
Teaching Hours:9
Enzyme Technology
 

Introduction, Enzymes Vs catalysts, Enzymes Vs Whole cells, General steps in production of enzymes, Selection criteria Immobilization of enzymes, Methods of Immobilization, Advantages of Immobilization, industrial applications of enzymes (leather, textile, baking, detergent industries), Biosensors, Types of Biosensors (Amperometric, Potentiometric, Conductimetric, Optical Biosensors), Immobilized enzymes in drug delivery.

Unit-7
Teaching Hours:6
Microbial Products
 

Classification of metabolic products- Primary, secondary and bioconversion products (Steroids). Production of alcoholic beverage (Beer, wine), Food (Cheese) amino acid (glutamic acid,) Organic acids (Lactic acid), antibiotic (penicillin) single cell protein, single cell oil. 

Unit-8
Teaching Hours:5
Quality Analysis and Regulations
 

Quality Control, Quality assurance, Standard Operating Procedures (SOP) & Good Manufacturing Practices (GMP) Social and Moral aspects of Biotechnology -Biotechnology and International trade - Patenting and commercial production of Biotechnology products, Regulations on use and distribution of Biotechnology products.

Text Books And Reference Books:

U. Sathyanarayana. Biotechnology. Books and Allied (P) Ltd, Kolkota: 2008.

S.N. Jogdand. Environmental Biotechnology. 3rded, India: Himalaya Publication House, 2001.

B.D. Singh. Biotechnology. 2nded, New Delhi: Kalyani Publishers, 2007.

 

Essential Reading / Recommended Reading

P. F. Stanbury. A. Whitaker and S.J. Hall. Principles of Fermentation Technology. 2nded, Edinburgh: Butterworth Heinemann Press, 2003

M. D. Pauline. Bioprocess Engineering Principles. 2nded, London: Academic Press, 2000.

Zhong, Jian-Jiang. Biomanufacturing. New York: Springer-Verlag Heidelberg, 2004

 

Evaluation Pattern

CIA I - 10 Marks

CIA 2 - 25 Marks

CIA 3 - 10 Marks

End Sem Exam - 50 Marks

Attendence - 5 Marks

MBTY351 - PRACTICAL IN IMMUNOLOGY AND BIOPROCESS ENGINEERING (2018 Batch)

Total Teaching Hours for Semester:120
No of Lecture Hours/Week:8
Max Marks:100
Credits:4

Course Objectives/Course Description

 

Students are imparted with the practical knowledge of various immunotechniques as well as using a living cell for production of industrially important products. This paper deals with production of wine, citric acid, and Enzymes from microbial source.

Learning Outcome

Students learn the practical skills such as media formulation sterilization, maintaining the pure culture of microbes, monitoring the growth curve of microbes. Students learn to perform assay for the production metabolites and enzymes.

 

Unit-1
Teaching Hours:120
PRACTICAL IN IMMUNOLOGY AND BIOPROCESS ENGINEERING
 

Immunology

1.      Determination of Blood group and Coomb’s test

2.      Total count of RBC & WBC using Haemocytometer      

3.      Differential Count of WBC                  

4.      Widal test and VDRL

5.      Dot ELISA

6.      Ouchterlony Double Diffusion

7.      Radial Immunodiffusion

8.      Rocket Immunoelectrophoresis

9.      Immunoblotting technique

10.  Osmotic fragility of RBC

Bioprocess Engineering

1.      Growth Kinetics of E. coli 

2.      Production of primary and secondary metabolite (organic acid)

3.      Estimation of the fermentation products by titration method

4.      Solid state Fermentation

5.      Production of beverage

6.      Immobilization of microbial cells and assay for its activity

7.      Isolation and assay for specific activity of amylase from Aspergillus niger.

8.      Isolation and screening of microorganism producing proteases

9.      Isolation and screening of microorganism producing amylases

10.  Isolation and mass production of Nitrogen fixers(Rhizobium)

11.  Visit to research institute or industry

 

 

Text Books And Reference Books:

1

U. Sathyanarayana. Biotechnology. Books and Allied (P) Ltd, Kolkota: 2008.

2

S.N. Jogdand. Environmental Biotechnology. 3rded, India: Himalaya Publication House, 2001.

3

B.D. Singh. Biotechnology. 2nded, New Delhi: Kalyani Publishers, 2007.

Essential Reading / Recommended Reading

1

P. F. Stanbury. A. Whitaker and S.J. Hall. Principles of Fermentation Technology. 2nded, Edinburgh: Butterworth Heinemann Press, 2003

2

M. D. Pauline. Bioprocess Engineering Principles. 2nded, London: Academic Press, 2000.

3

Zhong, Jian-Jiang. Biomanufacturing. New York: Springer-Verlag Heidelberg, 2004.

Evaluation Pattern

The evaluation will be based on performance, record, mid semester practical exams which are included in the CIA and End semester practical examination  
   
   

MBTY352 - PRACTICAL IN PLANT BIOTECHNOLOGY AND ANIMAL BIOTECHNOLOGY (2018 Batch)

Total Teaching Hours for Semester:120
No of Lecture Hours/Week:8
Max Marks:100
Credits:4

Course Objectives/Course Description

 

Students are imparted with the practical knowledge of using a living cell for production of industrially important products. This paper deals with production of wine, citric acid, and Enzymes from microbial source. The paper also deals with requirements of animal cell culture lab, different types of media preparation, initiation of cell culture and maintenance of cells. Cell viability assays are also included to get better acquainted with microscopy techniques which are of great significance in cell culture studies.

Learning Outcome

Students learn the practical skills such as media formulation sterilization, maintaining the pure culture of microbes, monitoring the growth curve of microbes. Students learn to perform assay for the production metabolites.

Unit-1
Teaching Hours:120
PRACTICAL IN PLANT BIOTECHNOLOGY AND ANIMAL BIOTECHNOLOGY
 

 Plant Biotechnology

1

Introduction to Plant Tissue Culture Laboratory
Nutritional components of Tissue Culture Media

2

Introduction to aseptic techniques
Introduction to different types of explants
Surface sterilization techniques
Callus initiation and Maintenance

3

Initiation of Cell suspension cultures and Growth kinetic studies using PCV/fresh and Dry weight basis

4

Induction of multiple shoots by using different explants

5

Culturing of anthers for the induction of haploids

6

Isolation and Culturing of protoplast

7

Induction of hairy roots with Agrobacterium rhizogenes infection using different explants and confirmation of transgene using PCR

8

Production of Artificial seeds using sodium alginate

9

Induction of Somatic Embryogenesis

Animal Biotechnology

10

Analysis of genetic diversity using RAPD markers

12

Set up of Animal Cell Culture Lab and Biosafety regulations

 

13

Preparation of animal cell culture media

 

14

Initiation of Primary Culture from Chick Embryo

 

15

Preparation of single cell suspension from spleen / liver / thymus

 

16

Cell counting and cell viability assessment by Trypan Blue method

 

17

Culture of lymphocytes

 

18

Cryopreservation of primary cultures and cell lines

 

19

Animal handling - techniques and regulations

 

20

Virus inoculation into chick/duck eggs for propagation

 

21

Estimation of viral load by ELISA.

 

22

Visit to Pasteur Institute, Coonoor

 

 

Text Books And Reference Books:

Rajan S and Christy R S.  Experimental Procedures in Life Sciences, India: Anjanaa Book House, Chennai, 2010.

S. Sadasivam and A. Manickam. Biochemical Methods. 3rd ed. India: New age International Publisher, 2008.

Essential Reading / Recommended Reading

Rajan S and Christy R S.  Experimental Procedures in Life Sciences, India: Anjanaa Book House, Chennai, 2010.

S. Sadasivam and A. Manickam. Biochemical Methods. 3rd ed. India: New age International Publisher, 2008.

Evaluation Pattern

The evaluation will be based on performance, record, mid semester practical exams which are included in the CIA and End semester practical examination

 

CIA Evaluation

Performance: 40 marks

Mid Semester Examination: 40 marks

Record: 20 marks

End Semester examination pattern:

1.      1. Conduct the experiment

(Total Marks 35 = Principle, procedure and graph, if any – 10, Working – 15, Result – 5, Comments/Interpretation - 5)

2.      2. Prepare the given explant for inoculation

(Total Marks 8 = Principle-2, Procedure-2, Working & Result: 4)                                                 

3.      3. Prepare synthetic seeds using the given plant material

(Total Marks 8 = Principle-2, Procedure-2, Working & Result: 4)                                                                            

4.      4. Submission of responding culture

  (Total Marks 8 = 2 X 4)                                                                                                                                    

5.     5. Logical questions

(Total Marks 6 = 2 x 3)                                                                                                                                                

6.     6. Spotters                                                                         

(Total Marks 10 = 4 x 2.5)

7. Animal Biotechnology Practical (25 marks)