CHRIST (Deemed to University), Bangalore

DEPARTMENT OF CHEMISTRY

School of Sciences

Syllabus for
Bachelor of Science (Biotechnology, Chemistry, Botany)
Academic Year  (2023)

 
3 Semester - 2022 - Batch
Course Code
Course
Type
Hours Per
Week
Credits
Marks
AEN321 ADDITIONAL ENGLISH Ability Enhancement Compulsory Courses 3 3 100
BOT331 PLANT TAXONOMY AND ECONOMIC BOTANY Core Courses 4 4 100
BOT351 PLANT TAXONOMY AND ECONOMIC BOTANY LAB Core Courses 4 2 50
BTY331 MOLECULAR-BIOLOGY AND BIOPHYSICS Core Courses 4 4 100
BTY351 MOLECULAR-BIOLOGY AND BIOPHYSICS LAB Core Courses 4 2 50
CHE331 CHEMISTRY III-ORGANIC AND ANALYTICAL CHEMISTRY Core Courses 4 4 100
CHE351 CHEMISTRY PRACTICALS - III Core Courses 2 2 50
ENG321 ENGLISH-III Ability Enhancement Compulsory Courses 3 2 100
FRN321 FRENCH Ability Enhancement Compulsory Courses 3 3 100
HIN321 HINDI Ability Enhancement Compulsory Courses 3 3 100
KAN321 KANNADA Ability Enhancement Compulsory Courses 3 03 50
SAN321 SANSKRIT Ability Enhancement Compulsory Courses 3 3 100
TAM321 TAMIL Ability Enhancement Compulsory Courses 3 3 100
4 Semester - 2022 - Batch
Course Code
Course
Type
Hours Per
Week
Credits
Marks
AEN421 ADDITIONAL ENGLISH - 3 3 100
BOT431 PLANT PHYSIOLOGY AND DEVELOPMENT - 4 4 100
BOT451 PLANT PHYSIOLOGY AND DEVELOPMENT LAB - 4 2 50
BTY431 GENETIC ENGINEERING - 4 4 100
BTY451 GENETIC ENGINEERING LAB - 4 2 50
CHE431 CHEMISTRY IV-INORGANIC AND PHYSICAL CHEMISTRY - 4 4 100
CHE451 CHEMISTRY PRACTICALS - IV - 2 2 50
ENG421 ENGLISH-IV - 3 2 100
FRN421 FRENCH - 3 3 100
HIN421 HINDI - 3 3 100
KAN421 KANNADA - 3 03 50
SAN421 SANSKRIT - 3 3 100
TAM421 TAMIL - 3 3 100
5 Semester - 2021 - Batch
Course Code
Course
Type
Hours Per
Week
Credits
Marks
BOT541A CELL BIOLOGY AND PLANT BREEDING Discipline Specific Elective Courses 3 3 100
BOT541B GENETICS, PLANT BREEDING AND EVOLUTION Discipline Specific Elective Courses 3 3 100
BOT542A ANALYTICAL TECHNIQUES IN PLANT SCIENCES Discipline Specific Elective Courses 3 3 100
BOT542B PHYTOCHEMISTRY AND PHARMACOGNOSY Discipline Specific Elective Courses 3 3 100
BOT542C ECONOMIC BOTANY AND PLANT RESOURCE UTILIZATION Discipline Specific Elective Courses 3 3 100
BOT551A CELL BIOLOGY AND PLANT BREEDING LAB Discipline Specific Elective Courses 4 2 50
BOT551B GENETICS, PLANT BREEDING AND EVOLUTION LAB Discipline Specific Elective Courses 4 2 50
BOT552A ANALYTICAL TECHNIQUES IN PLANT SCIENCES LAB Discipline Specific Elective Courses 4 2 50
BOT552B PHYTOCHEMISTRY AND PHARMACOGNOSY LAB Discipline Specific Elective Courses 4 2 50
BOT552C ECONOMIC BOTANY AND PLANT RESOURCE UTILIZATION LAB Discipline Specific Elective Courses 4 2 50
BTY541A IMMUNOLOGY Discipline Specific Elective Courses 3 3 100
BTY541B PLANT BIOTECHNOLOGY AND BIOINFORMATICS Discipline Specific Elective Courses 3 3 100
BTY542A BIOPROCESS ENGINEERING Discipline Specific Elective Courses 3 3 100
BTY542B FOOD SCIENCE Discipline Specific Elective Courses 3 3 100
BTY551A IMMUNOLOGY LAB Discipline Specific Elective Courses 4 2 50
BTY551B PLANT BIOTECHNOLOGY AND BIOINFORMATICS LAB Discipline Specific Elective Courses 2 2 50
BTY552A BIOPROCESS ENGINEERING LAB Discipline Specific Elective Courses 2 2 50
BTY552B FOOD SCIENCE LAB Discipline Specific Elective Courses 2 2 50
CHE531 CHEMISTRY V-PHYSICAL CHEMISTRY Core Courses 3 03 100
CHE541A CHEMISTRY VA-ORGANIC CHEMISTRY Discipline Specific Elective Courses 3 03 100
CHE541B CHEMISTRY VB-INORGANIC CHEMISTRY Discipline Specific Elective Courses 3 3 100
CHE551 CHEMISTRY PRACTICALS V-PHYSICAL CHEMISTRY Core Courses 2 02 50
CHE551A CHEMISTRY PRACTICALS VA-ORGANIC CHEMISTRY Discipline Specific Elective Courses 2 02 50
CHE551B CHEMISTRY PRACTICALS VB-INORGANIC CHEMISTRY Discipline Specific Elective Courses 2 2 50
6 Semester - 2021 - Batch
Course Code
Course
Type
Hours Per
Week
Credits
Marks
BOT641A PLANT BIOTECHNOLOGY AND BIOINFORMATICS - 3 3 100
BOT641B MOLECULAR BIOLOGY AND GENETIC ENGINEERING - 3 3 100
BOT642A HORTICULTURAL PRACTICES AND POST-HARVEST TECHNOLOGY - 3 3 100
BOT642B AQUATIC BOTANY - 3 3 100
BOT642C FORENSIC BOTANY AND WILDLIFE FORENSICS - 3 3 100
BOT651A PLANT BIOTECHNOLOGY AND BIOINFORMATICS LAB - 4 2 50
BOT651B MOLECULAR BIOLOGY AND GENETIC ENGINEERING LAB - 4 2 50
BOT652A HORTICULTURAL PRACTICES AND POST-HARVEST TECHNOLOGY LAB - 4 2 50
BOT652B AQUATIC BOTANY LAB - 4 2 50
BOT652C FORENSIC BOTANY AND WILDLIFE FORENSICS LAB - 4 2 50
BOT652D RESEARCH PROJECT IN BOTANY - 5 5 150
BTY631 ANIMAL BIOTECHNOLOGY - 3 3 100
BTY641A ALGAL BIOTECHNOLOGY - 3 3 100
BTY641B ENVIRONMENTAL BIOTECHNOLOGY - 3 3 100
BTY641C DEVELOPMENTAL BIOLOGY - 3 3 100
BTY641D HUMAN GENETICS - 3 3 100
BTY651 ANIMAL BIOTECHNOLOGY LAB - 4 2 50
BTY651A ALGAL BIOTECHNOLOGY LAB - 4 2 50
BTY651B ENVIRONMENTAL BIOTECHNOLOGY LAB - 4 2 50
BTY651C DEVELOPMENTAL BIOLOGY LAB - 4 2 50
BTY651D HUMAN GENETICS LAB - 4 2 50
BTY652E RESEARCH PROJECT IN BIOTECHNOLOGY - 5 5 150
CHE631 CHEMISTRY VI-MOLECULES OF LIFE - 3 3 100
CHE641A CHEMISTRY VIA-INDUSTRIAL MATERIALS AND ENVIRONMENT - 3 3 100
CHE641B CHEMISTRY VIB-CHEMISTRY OF NATURAL PRODUCTS AND HETEROCYCLIC COMPOUNDS - 3 3 100
CHE651 CHEMISTRY PRACTICALS VI-MOLECULES OF LIFE - 2 2 50
CHE651A CHEMISTRY PRACTICALS VIA-INDUSTRIAL MATERIALS AND ENVIRONMENT - 2 2 50
CHE651B CHEMISTRY PRACTICALS VIB-CHEMISTRY OF NATURAL PRODUCTS AND ORGANIC ANALYSIS - 2 2 50
CHE681 DISSERTATION IN CHEMISTRY - 7 5 100

AEN321 - ADDITIONAL ENGLISH (2022 Batch)

Total Teaching Hours for Semester:45
No of Lecture Hours/Week:3
Max Marks:100
Credits:3

Course Objectives/Course Description

 

Course Description

 

This course is taught in the second year for students from different streams, namely BA, BSc

 

and BCom. If the first year syllabus is an attempt by the Department of English, Christ

 

University to recognize and bring together the polyphonic Indian voices in English and Indian

 

regional literatures in translation for the Additional English students of the first year, the

 

second year syllabus intends to take that project a little further and open up the engagement

 

of the students to texts from across the world. The syllabus - selection of texts will

 

concentrate on readings from South Asian, Latin American, Australian, Canadian, and Afro-

 

American. It will voice subaltern concerns of identity, gender, race, ethnicity and problems of

 

belongingness experienced by humanity all over the globe.

 

The syllabus will extend the concerns of nation and nationality and marginalization,

 

discussed within the Indian context to a more inclusive and wider global platform. We have

 

consciously kept out ‘mainstream’ writers and concentrated on the voices of the subalterns

 

from across the world. There is an implicit recognition in this project that though the aspects

 

of marginalization and the problems facing subalterns are present across cultures and

 

nations, the experiences, expressions and reflections are specific to each race and culture.

 

The course will address these nuances and specificities and enable our students to become

 

more aware and sensitive to life and reality around them. This will equip the students, who

 

are global citizens, to understand not just the Indian scenario, but also situate themselves

 

within the wider global contexts and understand the spaces they will move into and negotiate

 

in their future.

 

There is a prescribed text book Blends: Voices from Margins for the second year students,

 

compiled by the Department of English, Christ University and intended for private circulation.

Course Objectives

 

The course objectives are

 

 to enable students to look at different cultures through Literature

 

 to help students develop an understanding of subaltern realities and identity politics

 

 to inculcate literary sensibility/taste among students across disciplines

 

 to improve language skills –speaking, reading, writing and listening

 

 to equip the students with tools for developing lateral thinking

 

 to equip students with critical reading and thinking habits

 

 to reiterate the study skills and communication skills they developed in the previous

 

year and extend it.

Course Outcome

CO1: it will enable students to understand and analyse the nuances of cultures, ethnicities and other diversity around them and become sensitive towards them.

CO2 : They will be able to critique literature from a cultural, ethical, social and political perspectives

Unit-1
Teaching Hours:12
Children?s Novel
 

TetsukoKuroyanagi: Tottochan: The Little Girl at the Window12

Unit-2
Teaching Hours:12
Short Story
 

Liliana Heker : “The Stolen Party

 

 Higuchi Ichiyo: “Separate Ways”

 

 Harukki Murakami "Birthday Girl"

 

 Luisa Valenzuela: “I’m your Horse in the Night”

 

Unit-3
Teaching Hours:12
Poetry
 

Poetry 12 Hrs

 

 Silvio Curbelo: “Summer Storm”

 

 Nancy Morejon: “Black Woman”

 

 Ruben Dario: “To Roosevelt”

 

 Mina Asadi: “A Ring to me is a Bondage”

Unit-4
Teaching Hours:9
Essay
 

Essay 9Hrs

 

 Amy Tan: “Mother Tongue

 

 Linda Hogan: “Waking Up the Rake”

 

 Isabelle Allande: “Open Veins of Latin America”

Text Books And Reference Books:

Blends Book II

Essential Reading / Recommended Reading

Oxford Encyclopeadia on Latin American History

Children's Literature -  Kimberley Reynolds (CUP)

Evaluation Pattern

Evaluation Pattern

 

CIA 1: A written test for 20 marks. It can be an Open Book test, a classroom assignment, an

 

objective or descriptive test pertaining to the texts and ideas discussed in class.

 

CIA2: Mid-semester written exam for 50 works

 

CIA 3: This is to be a creative test/ project in small groups by students. They may do

 

Collages, tableaus, skits, talk shows, documentaries, Quizzes, presentations, debates,

 

charts or any other creative test for 20 marks. This test should allow the students to explore

 

their creativity and engage with the real world around them and marks can be allotted to

 

students depending on how much they are able to link the ideas and discussions in the texts

 

to the world around them.

 

Question Paper Pattern

 

Mid Semester Exam: 2 hrs

 

Section A: 4x5= 20

 

Section B: 2x15=30

 

Total 50

 

End Semester Exam: 3 hrs

 

Section A: 4 x 5 = 20

 

Section B: 2 x 15= 30

 

Total 50

BOT331 - PLANT TAXONOMY AND ECONOMIC BOTANY (2022 Batch)

Total Teaching Hours for Semester:60
No of Lecture Hours/Week:4
Max Marks:100
Credits:4

Course Objectives/Course Description

 

This course describes theory, methods and practice of the taxonomy, classification and economic importance of flowering plants using vegetative and floral structures of 20+ families growing in Karnataka, India. The course describes the various classification systems and identification methods for angiosperm identification. In addition course describes the economic significance of various important commercial crops.

Course Outcome

CO1: Classify plant species and learn to identify the plants to their systematic position

CO2: Construct the taxonomic evidence from palynology, cytology, phytochemistry and molecular data

CO3: Analyze the principles and rules of botanical nomenclature

CO4: Determine the significance of economically important plants

CO5: Adapt the techniques of collection, preservation and processing of herbarium specimens

Unit-1
Teaching Hours:2
Introduction to plant taxonomy
 

Identification, Classification, Nomenclature

Unit-2
Teaching Hours:4
Identification
 

Functions of Herbarium, important herbaria and botanical gardens of the world and India; Documentation: Flora, Keys: single access and multi-access

Unit-3
Teaching Hours:4
Taxonomic evidences from palynology, cytology, phytochemistry and molecular data.
 

Taxonomic evidences from palynology, cytology, phytochemistry and molecular data.

Unit-4
Teaching Hours:5
Botanical nomenclature
 

Principles and rules (ICN); ranks and names; binomial system, typification, author citation,valid publication, rejection of names, principle of priority and its limitations.

Unit-5
Teaching Hours:2
Classification
 

Types of classification-artificial, natural and phylogenetic.Bentham and Hooker (upto series), Engler and Prantl (upto series).

Unit-6
Teaching Hours:4
Biometrics, numerical taxonomy and Phylogenic studies
 

Characters; variations; OTUs, character weighting and coding; cluster analysis; phenograms, Important phylogenetic terms and concepts: Plesiomorphic and Apomorphic characters; Homology and Analogy; Parallelism and Convergence; clades, Monophyly, Paraphyly and Polyphyly, origin & evolution of angiosperms, construction of cladogram.

Unit-7
Teaching Hours:6
Morphology
 

Part I Leaf Morphology (types, venation, phyllotaxy),

Part II Morphology of flower

a)      Parts of a flower- description of flower and its parts in technical terms.

b)      Types of flower – Hypogyny, Perigyny and Epigyny, Symmetry of flowers.

c)      Aestivation types.

d)      Placentation types.

e)      Floral Diagram and Floral Formula.

Part III Inflorescence:

a)    Racemose types-Simple Raceme, Corymb, Umbel, Spike, Spadix and Head.

b)    Cymose types-Simple Cyme, Monochasial- Scorpoid and Helicoid, Dichasial

c)      Special type- Cyathium, Hypanthodium

Part IV Fruits:

a)      Dry- dehiscent, indehiscent.

Fleshy - Simple,Aggregate, Multiple: Sorosis and Syconus

Unit-8
Teaching Hours:25
Family studies
 

Study the following families of Bentham and Hooker’s System with special reference to their morphological and floral characters. Special attention should be given to common and economically important plants within the families

Anonaceae, Brassicaceae, Malvaceae, Rutaceae, Leguminosae (Mimosaceae, Caesalpiniaceae and Fabaceae), Myrtaceae, Cucurbitaceae, Apiaceae, Rubiaceae, Compositae (Asteraceae), Apocynaceae, Asclepiadaceae, Solanaceae, Convolvulaceae, Acanthaceae, Verbenaceae, Lamiaceae (Labiatae), Euphorbiaceae, Moraceae, Liliaceae, Orchidaceae, Musaceae, Cannaceae, Graminae (Poaceae)

Unit-9
Teaching Hours:4
Economic Botany: Origin of Cultivated Plant
 

Concept of centers of origin, their importance with reference to Vavilov’s work

Unit-10
Teaching Hours:4
Study of the following groups of plants based on their uses with special reference to the botanical name, family and morphology of the useful part
 

Cereals: Rice, Wheat -Origin, morphology, uses

Millet: Ragi

Legumes: General account with special reference to Gram and soybean

Fruits: Apple, Orange and Banana

Vegetables- Bittergourd, Ladies finger, Carrot and Cabbage.

Flowers- commercial flowers

Plantation crops- coconut, coco, arecanut

Industrial crops-Rubber

Spices : General account with special reference to clove and black pepper (Botanical name, family, part used, morphology and uses)

Beverages: Tea (morphology, processing, uses), coffee

Oils and Fats: General description with special reference to groundnut

Fibre Yielding Plants: General description with special reference to Cotton (Botanical name, family, part used, morphology and uses)

Text Books And Reference Books:

1.Bhattacharya et.al. A Textbook of Botany, Vol.2. New Delhi: NCBA, 2013.

2. B. P Pandey, A textbook of botany: Angiosperms taxonomy, anatomy, embryology (including tissue culture) and economic botany, New Delhi: S Chand, 2009.

Essential Reading / Recommended Reading
  1. Henry and Chandra Bose, An Aid to the International Code of Botanical Nomenclature. Coimbatore: Botanical Survey of India, 2001.
  2. Jain S. K.  A Manual Of Ethnobotany, India: Scientific Publishers, 2004
  3. Pandey &Misra, Taxonomy of Angiosperms. New Delhi: Ane Book Pvt. Ltd. 2008
  4. Sivarajan V.V. Introduction to the Principles of Plant taxonomy, New Delhi: Oxford IBH Publishing Co. Pvt. Ltd., 1991
  5. Sreemali J.L. Economic Botany. Allahabad: KitabMahal. 1979.
  6. Swain T. Chemical Plant Taxonomy. New York: Academic Press. 1963.
  7. Verma.V. Text book of Economic Botany, Anne Book Pvt. Ltd., 2005.
  8. B. P Pandey, Taxonomy of Angiosperms, New Delhi: S Chand, 2005.
Evaluation Pattern

Continuous Internal Assessment (CIA)

 CIAI – Assignments/test/presentation/etc – 10%

 CIAII – Midsemester exam – 25%

 CIAIII - Assignments/test/presentation/etc – 10%

 Attendance – 5%

 End Semester Theory Exam – 50%

 

BOT351 - PLANT TAXONOMY AND ECONOMIC BOTANY LAB (2022 Batch)

Total Teaching Hours for Semester:60
No of Lecture Hours/Week:4
Max Marks:50
Credits:2

Course Objectives/Course Description

 

1) To acquaint with the aims, objectives and significance of taxonomy.

2) To identify the common species of plants growing in Karnataka and their systematic position.

3) To develop inductive and deductive reasoning ability.

4) To acquaint with the basic technique in the preparation of herbarium.

5) To familiarize with the plants having immense economic importance.

6) To enable the students to identify the plants especially medicinal and ornamental plants.

Course Outcome

CO1: Understand the vegetative and floral parts of the plant

CO2: Assess the significance of economically important plants and its parts.

CO3: Adapt the techniques of collection, preservation and processing of herbarium specimens

Unit-1
Teaching Hours:60
PLANT TAXONOMY AND ECONOMIC BOTANY LAB
 

1.      Identify the following inflorescence and fruits:-

a.       Inflorescence - Simple raceme, Spike, Corymb, Head, Dichasial cyme and       Cyathium.

b. Fruits - Simple: - Nut, Legume, Berry and Drupe;   Multiple and Aggregate

2.      Study of vegetative and floral characters of the following families (Description, V.S. flower, section of ovary, floral diagram/s, floral formula/e and systematic position according to Bentham & Hooker’s system of classification):

Anonaceae, Brassicaceae, Malvaceae, Rutaceae, Leguminosae (Mimosaceae, Caesalpiniaceae and Fabaceae), Myrtaceae, Cucurbitaceae, Apiaceae, Rubiaceae, Compositae (Asteraceae), Apocynaceae, Asclepiadaceae, Solanaceae, Convolvulaceae, Acanthaceae, Verbenaceae, Lamiaceae (Labiatae), Euphorbiaceae, Moraceae, Liliaceae, Orchidaceae, Musaceae, Cannaceae, Graminae (Poaceae)

3.      Identify the plants belonging to any 4 families mentioned in the syllabus upto genus by using the Flora.

4.      Students must describe the floral parts, draw the L.S., floral diagram and write the floral formula of at least one flower from each family.

5.      Study the finished products of plants mentioned in the syllabus of economic botany with special reference to the morphology, botanical name and family.

6.      Prepare herbarium of 10 plants with field notes.

7.   Workout nomenclatural problems regarding priority and author citations.

8.      Conduct field work under the guidance of a teacher

9.      Mounting of a properly dried and pressed specimen of any 10 wild plants with herbarium label (to be submitted during exam).

Text Books And Reference Books:

1.Bhattacharya et.al. A Textbook of Botany, Vol.2. New Delhi: NCBA, 2013.

2. B. P Pandey, A textbook of botany: Angiosperms taxonomy, anatomy, embryology (including tissue culture) and economic botany, New Delhi: S Chand, 2009.

Essential Reading / Recommended Reading
  1. Henry and Chandra Bose, An Aid to the International Code of Botanical Nomenclature. Coimbatore: Botanical Survey of India, 2001.
  2. Jain S. K.  A Manual Of Ethnobotany, India: Scientific Publishers, 2004
  3. Pandey &Misra, Taxonomy of Angiosperms. New Delhi: Ane Book Pvt. Ltd. 2008
  4. Sivarajan V.V. Introduction to the Principles of Plant taxonomy, New Delhi: Oxford IBH Publishing Co. Pvt. Ltd., 1991
  5. Sreemali J.L. Economic Botany. Allahabad: KitabMahal. 1979.
  6. Swain T. Chemical Plant Taxonomy. New York: Academic Press. 1963.
  7. Verma.V. Text book of Economic Botany, Anne Book Pvt. Ltd., 2005.
  8. B. P Pandey, Taxonomy of Angiosperms, New Delhi: S Chand, 2005.
Evaluation Pattern

Continuous Internal Assessment (CIA)

CIA-I – Performance – 20 Marks

CIA-II- Mid Semester Practical Examination – 20 Marks

CIA-III – Record – 10 Marks

 ESE - 50 Marks

BTY331 - MOLECULAR-BIOLOGY AND BIOPHYSICS (2022 Batch)

Total Teaching Hours for Semester:60
No of Lecture Hours/Week:4
Max Marks:100
Credits:4

Course Objectives/Course Description

 

The paper introduces the students with the fundamentals of molecular biology and biophysics. It also gives a clear out look on the molecular biology techniques used in the laboratory.

Course Outcome

CO1: Examine the basic concepts of molecular biology with depth understanding of cellular machinery.

CO2: Illustrate the structural and functional aspects of basic biomolecules such as DNA, RNA and protein and the mechanisms of DNA replication, transcription, translation.

CO3: Explain how gene expression is regulated.

CO4: Relate the molecular basis of life and the underlying genetic principles.

CO5: Describe the principle and applications of various bio analytical techniques.

Unit-1
Teaching Hours:4
Introduction to Macromolecules and Molecular Biology
 

Nucleic Acids:- DNA and RNA as genetic material and the proof (Griffith Experiment, Avery-McCarthy-McCleod Experiment, Hershey Chase Experiment, Biochemical evidences, Experiments using HRV and TMV )

Unit-2
Teaching Hours:15
DNA Structure, Properties and Replication
 

Watson and Crick model of DNA (Structure of Bases, Nucleosides and nucleotides, Chargaff Rule, Watson and Crick base pairing, Hoogsteen base pairing, physical measurements of DNA, antiparallel nature), Different forms for DNA (A, B and Z), chemical and spectroscopic properties of DNA (Effect of temperature:- denaturation and renaturation kinetics, Absorption of UV light, density gradient centrifugation, intercalating agents, effects of Acid and Alkali on DNA, solubility of DNA), DNA supercoiling (negative and positive supercoiling), Topoisomerase (Types and mechanisms). Hypothesis on DNA replication, Proof for Semi conservative model of replication of DNA (Meselson and Stahl Experiment, Thymidine incorporation Assay), Polarity of DNA replication, Prokaryotic DNA Replication Machinery: Gyrase, helicase, DNA polymerases (types, functions, properties) Origin of replication of DNA, Primer, Growing Fork, Mechanism of DNA replication (initiation, elongation (lagging and leading strand synthesis) and termination. Eukaryotic DNA replication – Multiple origins of replication, enzymes and proteins involved in replication, End replication problem and its solution (Telomere and telomerases and its significance in replication and involvement in cancer and aging). Models of DNA replication: Theta model and Rolling circle model, D-loop method. Inhibitors of DNA replication.

Unit-3
Teaching Hours:11
Alteration of Genome in Prokaryotes
 

Mutation: Definition and Types (Point mutation: Substitution, Addition, Deletion; Frame-shift Mutation, Missense and nonsense mutation, forward and reverse mutation, suppression mutation. Somatic and germline mutation, Transition and transversion, Neutral nonsynonymous and synonymous mutation, lethal mutation) causes of mutation: Spontaneous (Wobble base pairing, addition and deletion by DNA looping out, spontaneous chemical changes: oxidative damage, alkylation and deamination) and Induced mutations (UV, base analogues, alkylating, Hydroxylating and deaminating agents. Repair mechanisms - photoreactivation, Mismatch repair, excision repair (BER and NER), SOS repair and recombination repair, Transformation – Tatum and Lederberg’s experiment, Conjugation – F+ and F- strains, Hfr strains. Transduction - Lytic and lysogenic life cycles of bacteriophage, generalized and specialized transduction.

Unit-4
Teaching Hours:4
Genome Organization
 

Organization of genomes in prokaryotes and eukaryotes- concept of Gene, structure of genes, monocistronic and polycistronic genes, C value paradox, Gene organization and expression in mitochondria and chloroplast, functions, significance, role in evolutionary studies. Transposable elements – classes, transposons in bacteria, maize and Drosophila, retrotransposons, LINEs and SINEs, transposons and mutations.

Unit-5
Teaching Hours:8
RNA Structure, Function and Synthesis
 

Structure and functions of mRNA, tRNA, rRNA, snRNA, hnRNA, long noncoding RNA (LNC RNA) miRNA and siRNA. Ribozymes: Types of ribozymes, role of RNA in ribosome catalytic core). Types of RNA polymerases in prokaryotes and Eukaryotes. Sigma factors and subunits of RNA polymerase in prokaryotes. Transcription – initiation, elongation, and termination. Structure of promoter, initiation factors. Elongation factors and mechanism. Termination, types of termination: ATP dependent and independent, transcription factors and its importance, post transcriptional modifications of eukaryotic mRNA: capping and polyA tailing. RNA splicing. Introduction to CRISPR-Cas9: guide RNA.

Unit-6
Teaching Hours:7
Protein Synthesis and Modification
 

Properties of Genetic code and Wobble hypothesis. Mechanism of translation in prokaryotes and eukaryotes (activation and attachment of amino acid to tRNA, initiation, elongation and termination of polypeptide chain), role of Ribosomes in Protein synthesis, post translational modifications of proteins- (glycosylation, protein folding, acetylation, phosphorylation), polysomes.

Unit-7
Teaching Hours:6
Regulation of Gene Expression in Prokaryotes and Eukaryotes
 

Operon concept and its advantages, anabolic (trp operon) and catabolic operon (lac operon), Regulation of Gene expression in Eukaryotes -DNA (methylation) and Histone (acetylation) modifications.

Unit-8
Teaching Hours:5
Principles and Applications of Bioanalytical Techniques
 

Chromatography (Paper, Thin layer, column and GLC). Spectroscopy (Visible, Fluorescence, UV), Flow cytometry.

Text Books And Reference Books:

1) G. M. Malacinski, Freifelder's Essentials of Molecular Biology, 4th ed. New Delhi: Narosa Publications, 2013.

2) K. Wilson and J. Walker, Principles and Techniques of Biochemistry and Molecular Biology, 7th ed. New York: Cambridge University Press, 2010.

3) B. Lewin. Genes IX. Massachusetts: Jones and Bartlett Publishers, 2007.

Essential Reading / Recommended Reading

1) H. Lodish, A. Berk, P Matsudaira, C. A. Kaiser, M. Krieger, M. P. Scott, L. Zipursky and J, Darnell, Molecular Biology of the cell. 5th ed. New York: W.H. Freeman and company, 2008.

2) D.L. Nelson and M. M. Cox. Lehninger’s Principles of Biochemistry, 6th ed. USA. W. H. Freeman and company. 2013.

3) D. Voet and J. G. Voet. Biochemistry. 4th ed. USA: Wiley. 2011.

4) M. L. Srivastava. Bioanalytical Techniques, New Delhi: Narosa Publications. 2011.

5) J. D. Watson, T. A. Baker, S.P. Bell, A. Gann, M. Levine and R. Losick, Molecular biology of gene, 7th ed. USA: Cold Spring Harbor Laboratory Press, 2014.

6) R. M. J. Cotterill, Biophysics: An Introduction. New York: John Wiley & Sons, 2002.

7) B. R. Glick, J. J. Pasternak and C. L. Patten, Molecular Biotechnology: Principles and application of recombinant DNA. 4th ed. Washington DC: American Society for Microbiology Press, 2010.

Evaluation Pattern

Continuous Internal Assessment (CIA)

 CIAI – Assignments/test/presentation/etc – 10%

 CIAII – Midsemester exam – 25%

 CIAIII - Assignments/test/presentation/etc – 10%

 Attendance – 5%

 End Semester Theory Exam – 50%

BTY351 - MOLECULAR-BIOLOGY AND BIOPHYSICS LAB (2022 Batch)

Total Teaching Hours for Semester:60
No of Lecture Hours/Week:4
Max Marks:50
Credits:2

Course Objectives/Course Description

 

The papes aims at providing practical exposure to students with regards to the basic procedures in molecular biology lab. It deals with extraction and quantification of important biomolecules like DNA and protein.

Course Outcome

CO1: Demonstrate the extraction of proteins from animal and plant sources.

CO2: Illustrate the quantification and purity assessment of nucleic acids.

CO3: Plan estimation of DNA, RNA and protein.

CO4: Gain understanding of various bio analytical techniques.

CO5: Assess the importance of UV sterilization and the effect of UV on microbes

Unit-1
Teaching Hours:60
Name of the experiment
 

1) Preparation of Buffers-Citrate buffer, Phosphate buffer

2) Estimation of DNA by DPA method

3) Estimation of RNA by Orcinol method

4) Spectrophotometric estimation of DNA

5) Spectrophotometric estimation of RNA

6) Separation of amino acids by Paper Chromatography (ascending, descending and circular)

7) Separation of plant pigments by Thin Layer Chromatography and Paper chromatography

8) Extraction of protein from animal source by salt precipitation /organic solvent method & estimation using Biuret method

9) Extraction of protein from animal source by salt precipitation /organic solvent method & estimation using Lowry’s method

10) UV Mutagenesis

11) Ames test

 

Text Books And Reference Books:

1) S Sadasivam, A. Manickam. Biochemical Methods. 2 Ed, Delhi: New Age International Publishers Ltd, 1996.

2) S. K. Sawhney. R. Singh. Introductory Practical Biochemistry. New Delhi. Narosa Publications. 2014.

Essential Reading / Recommended Reading

1) M. L. Srivastava. Bioanalytical Techniques, New Delhi. India. Narosa Publications. 2011.

Evaluation Pattern

Continuous Internal Assessment (CIA)

CIA-I – Performance – 20 Marks

CIA-II- Mid Semester Practical Examination – 20 Marks

CIA-III – Record – 10 Marks

 ESE - 50 Marks

 

CHE331 - CHEMISTRY III-ORGANIC AND ANALYTICAL CHEMISTRY (2022 Batch)

Total Teaching Hours for Semester:60
No of Lecture Hours/Week:4
Max Marks:100
Credits:4

Course Objectives/Course Description

 

This course deals with the concepts of organic and analytical chemistry and builds the foundation for more advanced topics in the subsequent courses.

Course Outcome

CO 1: Summarise the fundamental aspects of organic molecules and their interactions.

CO 2: Justify the chemicals and reactions based on the green chemistry approach.

CO 3: Discuss the principles of analytical chemistry techniques and apply them in real sample analysis.

CO 4: Relate theory of separation techniques and instrumental methods for analysis.

Unit-1
Teaching Hours:8
Section A: Organic Chemistry 1. Organic Compounds of Nitrogen
 

 Prelearning topics: Classification and nomenclature of amines, Preparation of nitroalkanes and aromatic nitro compounds.

Amines (aliphatic and aromatic):  Preparation: From alkyl halides, Reduction of nitro compounds and nitriles, Reductive amination of aldehydes and ketones, Gabriel’s phthalimide synthesis, Hofmann bromamide reaction (with mechanism). Reactions: Hofmann (with mechanism) vs. Saytzeff elimination, Carbylamine test, Hinsberg test, with HNO2. Separation of a mixture of  1°, 2° and 3° amines using Hinsberg reagent. Structural features affecting basicity of aliphatic and aromatic amines. Comparative study of basicity of aliphatic and aromatic amines. Schotten – Baumann Reaction (with mechanism). Electrophilic substitution reactions of aniline: Halogenation, nitration and sulphonation.

Diazonium salts:  Preparation by diazotization.  Reactions: Conversion to benzene, phenol, iodo, fluoro and nitro benzene. Azo coupling.  Sandmeyer and Gatterman reactions.

 

 

Unit-2
Teaching Hours:5
2. Heterocyclic Compounds
 

Classification and nomenclature. Structure and aromaticity of 5-numbered and 6-membered rings containing one heteroatom. Synthesis and reactions of: Furan, Thiophene, Pyrrole, Pyridine, Indole, Quinoline  and Isoquinoline.

 

Unit-3
Teaching Hours:4
3. Introduction to Green Chemistry
 

Green Chemistry: Introduction - Environmental concern on chemical industry and need of green chemistry – Origin of green chemistry – Twelve principles of green chemistry with explanations - Atom economy and microwave assisted reactions - Green solvents . Microwave and ultrasound assisted green synthesis.

 

Unit-4
Teaching Hours:6
4. Polymers
 

 Introduction, types of polymers, polymerization reactions, Formation of Polythene, polypropylene, polystyrene, poly vinyl chloride, polyesters, polyamides including Nylon 6 and Nylon 6,6, resins.

Physical properties of polymers, molecular masses of polymers, Introduction to conducting polymers with examples. Environmental hazards of polymers, biodegradable polymers. Plastics, Recycling of plastics. Fibres: natural and synthetic, Rubbers: natural and synthetic.

 

Unit-5
Teaching Hours:7
5. Carbohydrates
 

Classification, and General Properties, Glucose (structural elucidation). Open chain and cyclic structures of fructose, galactose and mannose. Epimers and anomers. Determination of configuration of monosaccharides, Mutarotation, ascending and descending in monosaccharides. Interconversion of glucose and fructose. Structure of disacharrides (sucrose, maltose, lactose). Reducing and non-reducing sugars. polysacharrides (starch and cellulose) excluding their structure elucidation.

 

Unit-6
Teaching Hours:5
Section B: Analytical Chemistry 6. Statistical evaluation of analytical data
 

 Significant figures, Absolute error, accuracy, relative error, precision.

Classification of errors – (a) Determinate errors –Operational & Personal errors, Instrumental & reagent errors, Errors of method, Additive & proportional errors (b) Indeterminate or accidental errors.

Minimisation of errors– Calibration of apparatus & application of corrections, Running blank determination, Determination of accuracy of quantitative methods – Absolute method, Comparative method. Mean, median, standard deviation, variance (numerical problems)

 

Unit-7
Teaching Hours:8
7. Separation techniques
 

Solvent extraction Introduction– Classification– Principles and application of solvent extraction. Nernst’s distribution law, distribution co-efficient.

#Chromatography 

Introduction, Classification, Principles and Applications of column chromatography, thin layer chromatography, ion exchange chromatography, gas chromatography and high performance liquid chromatography (mention only).

 

Unit-8
Teaching Hours:13
8. Theory of chemical analysis
 

a) Qualitative analysis                                                                                                 5 Hrs

Introduction- Solubility product, ionic product, common ion effect, application of these in qualitative analysis. Selective precipitation of metal ions in their respective groups. Removal of interfering radicals.

b) Quantitative analysis                                                                                                8 Hrs

 Volumetric analysis: Introduction – Definition – Classification - Principles of acid base, redox, precipitation and complexometric titrations.

Theory of indicators (redox, acid base, metallochrome and adsorption indicators)

*Gravimetric analysis: Introduction –Classification – Principles. Organic reagents (DMG, Oxine) used for the precipitation.

 

 

Unit-9
Teaching Hours:4
9. Instrumental methods of analysis
 

Introduction ––Principles and application of spectrophotometry (colorimetry), Flame photometry

Electro analytical methods (potentiometry, conductometry).

 

Text Books And Reference Books:

[1] Bahl, A. & Bahl, B.S. Advanced Organic Chemistry, S. Chand, 2010.

[2] B. Mehta, M. Mehta, Organic Chemistry, PHI Learning Private Limited, 2017.

[3] D.A. Skoog, D.M. West, F.J. Holler and S.R. Crouch, Fundamentals of Analytical Chemistry, 8th Edition, Brooks/Cole, Thomson Learning, Inc., USA, 2004

 

 

 

Essential Reading / Recommended Reading

 [1]    Jain and Sharma Modern Organic Chemistry 3rd edition, Vishal Publishing Company, 2009.

 [2]    R. T Morrison and R. N. Boyd. Organic Chemistry. 7thed. New Delhi: Prentice-Hall of India (P) Ltd., 2010.

 [3]    S.M. Mukherji, S. P. Singh, and R. P. Kapoor. Organic Chemistry. 3rd, 12th Reprint, New Delhi: New Age International (P) Ltd. Publishers, 2009.

 [4]    I. L Finar, Organic Chemistry Vol. II, 5th ed. New Delhi: ELBS and Longman Ltd., reprint 2008.

 [5]    Vogels Textbook of Quantitative Chemical Analysis, 6th Edn., Pearson Education Ltd. 2009.

 

Evaluation Pattern

No.

Component

Schedule

Duration

Marks

CIA1

Assignment/quiz/group task/ presentations

Before MST

--

10

 

CIA2

Mid-Sem Test

[MST]

2 Hrs (50 marks)

25

CIA3

Assignment/quiz/group task/ presentations

After MST

--

10

CIA3

Attendance (75-79 = 1, 80-84 = 2, 85-89 = 3,

90-94 = 4, 95-100 = 5)

--

5

ESE

Centralized

3 Hrs (100 marks)

50

Total

100

CHE351 - CHEMISTRY PRACTICALS - III (2022 Batch)

Total Teaching Hours for Semester:30
No of Lecture Hours/Week:2
Max Marks:50
Credits:2

Course Objectives/Course Description

 

This course is intended to provide basic skills in qualitative analysis at the semi micro scale. Identification of cations and anions present in inorganic compounds has to be performed. Separation of sugar and amino acid mixtures can be achieved through chromatography.

 

 

 

Course Outcome

CO 1: Analyse inorganic salt mixtures.

CO 2: Discuss the separation of amino acid mixtures and sugar mixtures using chromatographic techniques.

Unit-1
Teaching Hours:25
Section A: Inorganic Chemistry
 

 Semi-micro qualitative analysis (using H2S or other methods) of mixtures - not more than four ionic species (two anions and two cations, excluding insoluble salts) out of the following:

    Cations : NH4+, Pb2+, Bi3+, Cu2+, Cd2+, Fe3+, Al3+ , Co2+ , Ni2+, Mn2+, Zn2+, Ba2+ , Sr2+ , Ca2+, K+

   Anions : CO32– , S2–, SO2, S2O32–, NO2, CH3COO, Cl, Br, I, NO3, SO42-, PO43-, BO33-

   (Spot tests should be carried out wherever feasible)

 

Unit-2
Teaching Hours:5
Section B: Organic Chemistry
 

 Separation of mixtures by Chromatography:

(a) Separation and identification of the components of a given mixture of two amino acids by paper chromatography/TLC

(b) Separation and identification of the components of a mixture of two sugars by paper chromatography/TLC

 

 

 

 

 

 

Text Books And Reference Books:

 [1] Svehla, G. Vogel’s Qualitative Inorganic Analysis, Pearson Education, 2012.

[2] Mann, F.G. & Saunders, B.C. Practical Organic Chemistry, 4th edition, Orient-Longman, 1979.

 

Essential Reading / Recommended Reading

[1] Textbook of Practical Organic Chemistry, Prentice-Hall, 5th edition, 1996.

Evaluation Pattern

 

No.

Component

Duration

Points

Marks

CIA1

Mid-Sem Test

3 Hrs

50

20

 

CIA2

Class work, PreLab Quiz, assignments

---

40

20

CIA3

Record book

-----

20

10

ESE

Centralized (two Examiners)              3 Hrs

 50

50

Total

25+25=50

ENG321 - ENGLISH-III (2022 Batch)

Total Teaching Hours for Semester:45
No of Lecture Hours/Week:3
Max Marks:100
Credits:2

Course Objectives/Course Description

 

 

Course Description

English is offered as a course for all the students in BA, BSc, BCom, and BBA F&A classes in the third and fourth semesters. The aim is to strengthen the communication skills, and particularly study skills of the learners further, through adequate practice and exposure to good examples of writing, thought, ideas and human values. In addition, they will be trained in study skills through tasks in academic genres such as message, letter, essay, data interpretation etc. It aims to not only equip learners with skills but also sensitize them towards issues that concern human life in today’s globalised context. The course content is selected to meet the requirements of the departmental goal of “empowering the individual to read oneself, the social context and the imagined”; institutional goal of ensuring “holistic development”; and the national goal of creating competent and valuable citizens. The primary objective of this course is to help learners develop appropriate employability skills and demonstrate suitable conduct with regards to communication skills. The units are organised in order to help the learners understand the academic and workplace demands and learn by practice.

 

Course Objectives     

 

 

·       To enable learners to develop reading comprehension for various purposes

 

·       To enable learners to develop writing skills for academic and professional needs

 

·       To enable learners to develop the ability to think critically and express logically

 

·       To enable learner to communicate in a socially and ethically acceptable manner

 

·       To enable learners, to read, write and speak with clarity, precision and accuracy

 

 

 

 

 

 

 

 

 

 

 

 

 

Course Outcome

CO1: Recognise the errors of usage and correct them. Recognize their own ability to improve their own competence in using the language

CO2: Read independently unfamiliar texts with comprehension. Read longer texts, compare, and evaluate them.

CO3: Understand the importance of writing in academic life. Write simple sentences without committing errors in spelling and grammar. Plan a piece of writing using drafting techniques.

Unit-1
Teaching Hours:10
Introduction to university grammar
 

 

Subject verb agreement

 

Tenses

 

Preposition

 

Voices

 

Clauses

 

Unit-2
Teaching Hours:10
Strategies for Reading
 

 

Skimming and scanning

 

Strategies of reading

 

Reading and understanding reports

 

Reading content/ texts of various kinds

 

Inferencing skills

 

Academic vocab

 

Academic phrases

 

Professional expression

 

Study skills- library and referencing skills (organising reading, making notes, managing time, prioritising)

 

Unit-3
Teaching Hours:10
Strategic writing for academic purpose
 

 

Mind mapping

 

Organising ideas

 

Accurate usage of vocabulary

 

Paragraph strategy

 

Cohesion and sequencing (jumbled sentences to paragraph)

 

Extended writing 

 

Formal and informal writing

 

Reports (all types including illustration to report and report to illustration and/or graphs, charts, tables and other statistical data)

 

Proposal writing (for projects, for research)

 

Academic essays/ articles

 

Persuasive writing, extrapolative writings

 

Case study writing

 

Executive summaries

 

Editing, proofreading skills

 

Resume vs CV

 

Unit-4
Teaching Hours:10
Listening and Oral communication
 

 

Self-introduction

 

Body language

 

Talks, speeches and presentations

 

Conversation

 

Telephone conversation

 

Meetings

 

Group discussion

 

Seminar / conference presentation

 

Unit-5
Teaching Hours:5
Business communication
 

 

Principles of communication

 

Process of communication

 

Types of communication

Barriers in communication

Text Books And Reference Books:

NIL

Essential Reading / Recommended Reading

ENGlogue -2

Evaluation Pattern

 

Evaluation Pattern

 

CIA 1: Classroom assignment/test/ written or oral tasks for 20 marks keeping in tune with the course objectives and learning outcomes.

CIA 2: Mid-semester exam for 50 marks.

CIA 3: Collage, tableaus, skits, talk shows, documentaries, Quizzes or any creative assignments.

 

 End- semester 50 marks 

 

End Semester Exam: 2 hrs